well method assay
Description
Figure 1. Plant extract preparation. Step 1 involves the maceration of plants, followed by Step 2, which illustrates the immersion of smashed samples in methanol. Subsequently, in Step 3, bacterial lawns were inoculated on Muller-Hinton agar. Step 4 showcases the performance of the agar well diffusion method, a crucial stage in evaluating antibacterial activity. Finally, Step 5 demonstrates the measurement of the Zone of Inhibition.
Acknowledgements
References
1. Bhatwalkar SB, Mondal R, Krishna SBN, Adam JK, Govender P, Anupam R. Antimicrobial Resistance Collaborators. (2022). Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis.
2. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999 Oct;12(4):564-82.. Dubreuil JD. Fruit extracts to control pathogenic Escherichia coli: A sweet solution. Heliyon.
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