Viral Plaque Assay Protocol
Description
This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication.
This figure describes a general protocol for performing a viral plaque assay. First, cells are grown to near confluency in a 12 well plate. Once cells reach confluency, media is removed and 50 μL of serially diluted virus is added to each well. Phosphate-buffered saline (PBS) is added to one well as a control. Following 1 hr incubation at 37°C and 5% CO2, 3 ml of growth media overlay agar is added to each well, and the wells are incubated for 48 hrs at 37°C and 5% CO2. Afterwards, 1 ml of 4% PFA is deposited onto the overlay and left for 1 hr at room temperature. Lastly, 1 ml of crystal violet is added to each well and incubated for 5 minutes. Wells can then be rinsed and observed.
Acknowledgements
References
Andersson, T. (2020) Plaque Assay: A Method to Determine Viral Titer as Plaque Forming Units (PFU). Journal. https://www.jove.com/science-education/10514/plaque-assay-method-to-determine-viral-titer-as-plaque-forming-units
Get started with this template for freeDon’t start from scratch.
Create professional scientific illustrations quickly and easily, even without any design expertise
- Get started with a huge library of editable icons and templates such as common biological pathways, anatomy, or genetics.
- Create figures that effectively communicate your research in half the time using our editable icons.
- Use our PDB tool to quickly generate and customize protein structures