Top-down Proteomics of Bacteria
Description
This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication.
A standard workflow for top-down proteomics of bacteria. First, sample is collected and bacteria are lysed to extract proteins. The bacterial lysate is then filtered to purify proteins which will be then be fractionated according to their molecular mass. Proteins inside each fraction are then separated by liquid chromatography (LC) and sequenced by tandem mass spectrometry (MS/MS). Proteins and their post-translational modifications are finally identified by database searches.
Acknowledgements
References
Ntai, A. et. al.. (2016) A Method for Label-Free, Differential Top-Down Proteomics. Methods Mol Biol.. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777307/
Toby, T. et. al.. (2016) Progress in Top-Down Proteomics and the Analysis of Proteoforms. Annual Review of Analytical Chemistry . https://www.annualreviews.org/doi/full/10.1146/annurev-anchem-071015-041550
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