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An editable high resolution scientific image depicting Three-dimensional (3D) cell culture systems

Three-dimensional (3D) cell culture systems

Description

Overview of scaffold-free techniques for 3D tissue generation.

Acknowledgements

References

S. R. Horman, J. To, A. P. Orth, N. Slawny, M. J. Cuddihy, and D. Caracino, “High-content analysis of three-dimensional tumor spheroids: investigating signaling pathways using small hairpin RNA,” Nat Methods, vol. 10, no. 10, pp. v–vi, Oct. 2013, doi: 10.1038/nmeth.f.370. R. Jeske et al., “Agitation in a microcarrier-based spinner flask bioreactor modulates homeostasis of human mesenchymal stem cells,” Biochem Eng J, vol. 168, p. 107947, Apr. 2021, doi: 10.1016/j.bej.2021.107947. R. Mitteregger, G. Vogt, E. Rossmanith, and D. Falkenhagen, “Rotary cell culture system (RCCS): a new method for cultivating hepatocytes on microcarriers.,” Int J Artif Organs, vol. 22, no. 12, pp. 816–22, Dec. 1999. W. Metzger et al., “The liquid overlay technique is the key to formation of co-culture spheroids consisting of primary osteoblasts, fibroblasts and endothelial cells,” Cytotherapy, vol. 13, no. 8, pp. 1000–1012, Sep. 2011, doi: 10.3109/14653249.2011.583233. S. Raghavan, P. Mehta, E. N. Horst, M. R. Ward, K. R. Rowley, and G. Mehta, “Comparative analysis of tumor spheroid generation techniques for differential in vitro drug toxicity,” Oncotarget, vol. 7, no. 13, pp. 16948–16961, Mar. 2016, doi: 10.18632/oncotarget.7659. D. Dhamecha, D. Le, T. Chakravarty, K. Perera, A. Dutta, and J. U. Menon, “Fabrication of PNIPAm-based thermoresponsive hydrogel microwell arrays for tumor spheroid formation,” Materials Science and Engineering: C, vol. 125, p. 112100, Jun. 2021, doi: 10.1016/j.msec.2021.112100. P. Benien and A. Swami, “3D Tumor Models: History, Advances and Future Perspectives,” Future Oncology, vol. 10, no. 7, pp. 1311–1327, May 2014, doi: 10.2217/fon.13.274. R.-Z. Lin, W.-C. Chu, C.-C. Chiang, C.-H. Lai, and H.-Y. Chang, “Magnetic Reconstruction of Three-Dimensional Tissues from Multicellular Spheroids,” Tissue Eng Part C Methods, vol. 14, no. 3, pp. 197–205, Sep. 2008, doi: 10.1089/ten.tec.2008.0061.
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