Targeted CRISPR CAS9 screen
Description
Cells are being nucelofected in 96 well format. Cell viability is monitored based on GFP+ expression over time. Essential genes (eg sgRPL17) show a reduction in cell viability aka GFP+ expression whereas GFP+ expression and cell viability will remain stable in non-essential genes (eg sgAAVS1)
Acknowledgements
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