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This figure template "Sandwich ELISA" is assembled using dynamic BioRender assets (icons, lines, shapes and/or text) and is fully editable. To customize your own personalized figure using this template as a starting point, click "Use Template". Sandwich ELISA is an immunoassay technique used to detect and quantify antigens or antibodies in a sample. This method involves immobilizing a capture antibody on the surface of a microtiter plate, which specifically binds to the antigen of interest. A primary antibody is then added to the well, which binds to another epitope on the antigen and creates a "sandwich" between two antibodies. A detection antibody conjugated to biotin is then added to bind the primary antibody. Streptavidin-HRPO then binds biotin, and catalyzes a chromogenic substrate to produce a signal that is proportional to the concentration of the antigen. [Template description adapted from GPT3.5, 2023]