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This figure template "RNA Sequencing" is assembled using dynamic BioRender assets (icons, lines, shapes and/or text) and is fully editable. To customize your own personalized figure using this template as a starting point, click "Use Template". RNA Sequencing is a powerful method for quantifying the sequences of RNA molecules in a sample, allowing a comprehensive view of the transcriptome. The template illustrates the main steps of RNA-seq which are: isolation of RNA from a sample, fragmentation of RNA into small pieces, conversion of RNA into cDNA, sequencing the cDNA fragments using NGS, and aligning the sequence data to a reference genome to quantify transcripts. [Template description adapted from GPT3.5, 2023]