Primer Extension Inhibition Assay/ Toeprinting
Description
The toeprinting assay, also known as the primer extension inhibition assay, is a technique used in molecular biology to investigate the interactions between messenger RNA, ribosomes, and RNA-binding proteins. Unlike the commonly used DNA footprinting assay, this approach helps to examine the formation of the translation initiation complex.
Acknowledgements
References
Timothy W. Nilsen
Adapted from RNA: A Laboratory Manual, by Donald C. Rio, Manuel Ares Jr, Gregory J. Hannon, and Timothy W. Nilsen. CSHL Press, Cold Spring Harbor, NY, USA, 2011.
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