Parse Evercode Split Pool Combinatorial Single Cell Barcoding
Description
Overview of Evercode Combinatorial Barcoding workflow.
Fixed single cells or nuclei undergo several rounds of split-pool barcoding to uniquely label transcripts according to cell of origin. Cells are then lysed, and barcoded transcripts undergo library preparation, sequencing, and data analysis. Single cell transcriptomes are constructed by grouping transcripts containing the same four barcode combinations.
Acknowledgements
References
Tran, Vuong & Papalexi, Efthymia & Schroeder, Sarah & Kim, Grace & Sapre, Ajay & Pangallo, Joey & Sova, Alex & Matulich, Peter & Kenyon, Lauren & Sayar, Zeynep & Koehler, Ryan & Diaz, Daniel & Gadkari, Archita & Howitz, Kamy & Nigos, Maria & Roco, Charles & Rosenberg, Alexander. (2022). High sensitivity single cell RNA sequencing with split pool barcoding. 10.1101/2022.08.27.505512.
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