Oocyte Expression Studies Of The Human α7 nAChR Subunit

Description

Site-directed mutagenesis was performed using the QuikChange Mutagenesis Kit (Agilent Technologies). Female adult Xenopus laevis isolated oocytes were provided by the European Xenopus Resource Centre (Portsmouth, UK) and defolliculated as described previously (Young et al., 2007)

Acknowledgements

References

ROADBENT, S., GROOT-KORMELINK, P. J., KRASHIA, P. A., HARKNESS, P. C., MILLAR, N. S., BEATO, M. & SIVILOTTI, L. G. 2006. Incorporation of the beta3 subunit has a dominant-negative effect on the function of recombinant central-type neuronal nicotinic receptors. Mol Pharmacol, 70, 1350-7. YOUNG, G. T., BROAD, L. M., ZWART, R., ASTLES, P. C., BODKIN, M., SHER, E. & MILLAR, N. S. 2007. Species selectivity of a nicotinic acetylcholine receptor agonist is conferred by two adjacent extracellular beta4 amino acids that are implicated in the coupling of binding to channel gating. Mol Pharmacol, 71, 389-97.

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