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This figure template "One-step vs. Two-step RT-PCR" is assembled using dynamic BioRender assets (icons, lines, shapes and/or text) and is fully editable. To customize your own personalized figure using this template as a starting point, click "Use Template". This templates visualizes the comparison between one-step and two-step RT-PCR. One-step PCR offers a simpler and more straightforward technique, as reverse transcription and PCR is performed in one step. With two-step RT-PCR, reverse transcription and PCR occurs in two distinct steps, which can be more time-consuming, complicated and costly. However, compared to the one-step RT-PCR, two-step RT-PCR can provide more accurate quantification of RNA and can detect low abundance transcripts. [Template description adapted from GPT3.5, 2023]