Neutral Red Uptake Assay
Description
The neutral red uptake assay quantitatively estimates the number of viable cells in a culture. It is one of the most used cytotoxicity tests with many biomedical and environmental applications. It is based on the ability of viable cells to incorporate and bind the supravital dye neutral red in the lysosomes.
Acknowledgements
References
Borenfreund, E. and Puerner, J. [1985] Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol. Lett. 24:119-124.
Repetto G, Del Peso A, Zurita JL. Neutral red uptake assayfor the estimation of cell viability/cytotoxicity. Nat Prot. 2008;3:1125–31.
Ndhlala AR, Anthonissen R, Stafford GI, Finnie JF, Verschaeve L, Van Staden J. In vitro cytotoxic and mutagenic evaluation of thirteen commercial herbal mixtures sold in KwaZulu-Natal, South Africa. S Afr J Bot. 2010;76:132–8.
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