Origin and Application of iPSCs

Description

For C9orf72 Hexanucleotide repeat expansion iPSCs to MNs requires 3 kits: NIM → NEM → MND → MNM NIM = Human ES/iPS Neural Induction Medium = Neural Rosettes NEM = ENStem™-A Neural Expansion Medium = Neural Progenitor MND = STEMdiff™ Motor Neuron Differentiation Kit = Motor Neurons MNM = STEMdiff™ Motor Neuron Maturation Kit = terminally differentiated MNs To ensure that we formed MNs = qPCR of markers linked to neuronal differentiation: SMI32 (NF-H), TUBB3 (TUJ), ISL1, NKX6.1, S100β and Nestin

Acknowledgements

References

STEMDIFFTM Motor Neuron differentiation kit. STEMCELL Technologies. (n.d.). https://www.stemcell.com/products/stemdiff-motor-neuron-differentiation-kit.html Thonhoff, J., Ojeda, L., & Wu, P. (2009). Stem cell-derived motor neurons: Applications and challenges in amyotrophic lateral sclerosis. Current Stem Cell Research & Therapy, 4(3), 178–199. https://doi.org/10.2174/157488809789057392

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