Malarial culturing in RBCs
Description
A protocol for preparing RBCs and culturing Plasmodium falciparum with RBCs and staining procedures
Acknowledgements
References
Jensen, J.B. In vitro culture of Plasmodium falciparum parasites. in Malaria Methods and Protocols (ed. Doolan, D.L.) 477–488 (Humana Press, Totowa, New Jersey, 2002).
Radfar, A., Méndez, D., Moneriz, C., Linares, M., Marín-García, P., Puyet, A., ... & Bautista, J. M. (2009). Synchronous culture of Plasmodium falciparum at high parasitemia levels. Nature protocols, 4(12), 1899-1915.
Duffy, S., & Avery, V. M. (2017). Plasmodium falciparum in vitro continuous culture conditions: A comparison of parasite susceptibility and tolerance to anti-malarial drugs throughout the asexual intra-erythrocytic life cycle. International Journal for Parasitology: Drugs and Drug Resistance, 7(3), 295-302.
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