Infection of mammalian followed by gene expression analysis using RT-qPCR and zymography assay
Description
MDA-MB-231, a triple negative breast cancer cell line were infected with Borrelia-B31 strain for 24, 48 and 72 hours. Total RNA was extracted followed by expression patterns of MMPs and TIMPs were assessed using Quantitative RT-qPCR. Further validation of target gene was done using zymography assay which involves collecting cell lysates and performing Bradford's protein estimation assay and estimation of activity of active enzymes in the samples.
Acknowledgements
References
1. Gaur G, Sawant JY, Chavan AS, et al. Effect of Invasion of Borrelia burgdorferi in Normal and Neoplastic Mammary Epithelial Cells. Antibiotics (Basel). 2021;10(11):1295. Published 2021 Oct 24. doi:10.3390/antibiotics10111295
Get started with this template for freeDon’t start from scratch.
Create professional scientific illustrations quickly and easily, even without any design expertise
- Get started with a huge library of editable icons and templates such as common biological pathways, anatomy, or genetics.
- Create figures that effectively communicate your research in half the time using our editable icons.
- Use our PDB tool to quickly generate and customize protein structures
.gif)
