In-Cell Western Assay
Description
This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication.
This assay protocol provides the basic steps for the in-cell western immunofluorescence assay. This protocol includes tissue culture growth, test compound treatment, primary and secondary antibody treatment, and scanning and analysis. This protocol can be applied to any experiments assessing the quantification of two distinct proteins.
Acknowledgements
References
Ma, H.W. et al. (2017) In-Cell Western Assays to Evaluate Hantaan Virus Replication as a Novel Approach to Screen Antiviral Molecules and Detect Neutralizing Antibody Titers. Front Cell Infect Microbiol. https://www.frontiersin.org/articles/10.3389/fcimb.2017.00269/full
Hoffman, G.R. et al. (2010) A High-Throughput, Cell-Based Screening Method for siRNA and Small Molecule Inhibitors of mTORC1 Signaling Using the In Cell Western Technique. Assay Drug Dev Technol. https://www.liebertpub.com/doi/10.1089/adt.2009.0213
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