Immunohistochemistry (IHC)
Description
Immunohistochemistry (IHC; in cells termed immunocytochemistry; with fluorescent reagents termed immunofluorescence) is a technique used to visualize the expression of proteins. In direct IHC, the primary antibody binds to the protein and is conjugated directly to a label (fluorophore) that can be visualized. Indirect IHC utilizes a secondary antibody (with fluorophores) that recognizes the primary antibody and amplifies the signal.
Acknowledgements
References
Carter, M., & Shieh, J. C. (2015). Guide to research techniques in neuroscience. Academic Press.
Get started with this template for freeDon’t start from scratch.
Create professional scientific illustrations quickly and easily, even without any design expertise
- Get started with a huge library of editable icons and templates such as common biological pathways, anatomy, or genetics.
- Create figures that effectively communicate your research in half the time using our editable icons.
- Use our PDB tool to quickly generate and customize protein structures