How to Break the Diffraction Limit with STORM
Description
This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication.
In microscopy, resolution is limited by diffraction. One way to circumvent this is using stochastic optical reconstruction microscopy (STORM). Diffraction is a law of physics; STORM cannot change that. However, by imaging fluorescent structures at individual spots, the centers of these spots can be determined. After many rounds, the centers (and thus precise locations) of all molecules are known and can be superimposed to render a super-resolved structure.
Acknowledgements
References
Rust, M. J. et al. (2006) Stochastic optical reconstruction microscopy (STORM) provides sub-diffraction-limit image resolution. Nature. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2700296/
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