His-tagged Protein Purification

Description

His-tagged Protein Purification is a method for purifying proteins by using a His tag, a sequence of histidine residues attached to the protein of interest. This technique employs metal affinity chromatography to efficiently isolate the desired proteins. Alternatively, GST tags can be used. The workflow protocol outlines the step-by-step procedure required for successful protein purification. [Template description adapted from GPT3.5, 2023]

Acknowledgements

References

https://www.sigmaaldrich.com/technical-documents/protocols/biology/affinity-chromatography-tagged-proteins/manual-purification-using-his-gravitrap-talon.html

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