Growing Norovirus in Human Intestinal Enteroids (HIEs)
Description
This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication.
This figure describes a protocol for growing human norovirus in human intestinal enteroids (HIEs). Briefly, 1) primary cells are collected from intestinal tissue and cultured as HIEs, which are 2) maintained weekly. 3) HIEs are trypsinized, strained, and used to seed monolayers. When monolayers are confluent, Wnt growth factor is removed in order to differentiate cells. 4) Monolayers are then infected in duplicate with norovirus, incubated to allow virus to bind, and washed. One monolayer is frozen and the second grown for 72 hours. Finally, norovirus RNA is extracted and analyzed using RT-qPCR to compare viral growth.
Acknowledgements
References
Ettayebi, K., et. al.. (2017) Replication of Human Noroviruses in Stem Cell-Derived Human Enteroids. Science. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5305121/
Get started with this template for freeDon’t start from scratch.
Create professional scientific illustrations quickly and easily, even without any design expertise
- Get started with a huge library of editable icons and templates such as common biological pathways, anatomy, or genetics.
- Create figures that effectively communicate your research in half the time using our editable icons.
- Use our PDB tool to quickly generate and customize protein structures
![example of using the icon library](https://cdn.prod.website-files.com/621e6f1effebfe03881da9bd/64c7c0fc322ef8c15744f235_ezgif.com-video-to-gif%20(1).gif)