Golden Gate cloning overview
Description
Golden Gate cloning is a DNA assembly method that uses Type IIS restriction enzymes, including BsaI and BpiI, that can be used to assemble transcriptional units from standard modular parts (i.e. promoters, terminators, and coding sequences), and assemble multigene constructs from a combination of the transcriptional units made (based on Engler et al., 2014).
Acknowledgements
References
Engler, C., Youles, M., Gruetzner, R., Ehnert, T.M., Werner, S., Jones, J.D., Patron, N.J., and Marillonnet, S. (2014). A golden gate modular cloning toolbox for plants. ACS Synth Biol 3, 839-843.
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