Generate Human Monocytic Dendritic Cells From Peripheral Blood
Description
This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication.
To generate human monocytic dendritic cells, peripheral blood is collected from healthy donors, layered on top of a density gradient cell separation medium, and centrifuged. White blood cells are collected and washed with PBS before counting peripheral blood mononuclear cells (PBMCs). Subsequently, CD14+ monocytes are isolated using magnetic separation and plated in a 6-well plate with 800 U/mL GM-CSF and 400 U/mL IL-4. Media is replenished as needed. Immature human monocytic-DCs can be collected on day 5 of culture.
Acknowledgements
References
Dauer, M et. al. (2003) Mature Dendritic Cells Derived from Human Monocytes Within 48 Hours: A Novel Strategy for Dendritic Cell Differentiation from Blood Precursors. J Immunol.
Kim, SJ et. al. (2019) Human CD141+ Dendritic Cells Generated From Adult Peripheral Blood Monocytes. Cytotherapy.
Get started with this template for freeDon’t start from scratch.
Create professional scientific illustrations quickly and easily, even without any design expertise
- Get started with a huge library of editable icons and templates such as common biological pathways, anatomy, or genetics.
- Create figures that effectively communicate your research in half the time using our editable icons.
- Use our PDB tool to quickly generate and customize protein structures