Gelatin Zymography
Description
Protocol workflow describing Gelatin Zymography, a technique that utilizes a modified SDS-polyacrylamide gel electrophoresis (PAGE) to assess the gelatinolytic activity of enzymes such as Matrix Metalloproteinases 2 and 9 (MMP-2 and MMP-9). The presence of bands shows hydrolytic activity influenced by conditions such as incubation time, protein quantity and protein quality. This method can be used to test gelatinase presence and activity in tissue/cell lysates and purified protein preps.
Acknowledgements
David Peeney
References
Toth, M., & Fridman, R. (2001). Assessment of Gelatinases (MMP-2 and MMP-9 by Gelatin Zymography. Methods in molecular medicine, 57, 163–174. https://doi.org/10.1385/1-59259-136-1:163
Vandooren, J., Geurts, N., Martens, E. et al. Zymography methods for visualizing hydrolytic enzymes. Nat Methods 10, 211–220 (2013). https://doi.org/10.1038/nmeth.2371
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