Gateway cloning
Description
Gateway cloning strategy. A: Schematic overview of the generation of the middle entry clone using a donor vector. B: Schematic overview of the 3-part LR recombination reaction for the generation of expression constructs, using three entry clones and a destination vector.
Acknowledgements
References
Kwan, Kristen M et al. “The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs.” Developmental dynamics : an official publication of the American Association of Anatomists vol. 236,11 (2007): 3088-99. doi:10.1002/dvdy.21343
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