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This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication. 1) Extracellular Vesicles (EV) are released by cells during their normal activity. 2) For EV separation, conditioned culture medium is harvested and major contaminants removed by consecutive low speed centrifugations. 3) The cleared supernatant is concentrated by ultracentrifugation, and the resulting 100K pellet is loaded on the bottom of an iodixanol gradient. EVs float upwards and EV-enriched fractions are collected and pelleted. The final sample is rich in EVs and absent in contaminants.