Experimental Protocol for Differentiation and Transfection of 3T3-L1 Cells
Description
The figure describes the experimental protocol for differentiating 3T3-L1 preadipocytes into mature adipocytes. The protocol consists of several steps:
1. Seeding the cells into 96-well plates 24h prior to transfection
2. Transfection of cells with DDS
3. Addition of induction medium to cells 24h after transfection to trigger the differentiation
4. Addition of differentiation medium on day 2 and day 4
5. ORO staining of lipid droplets in mature adipocytes
Acknowledgements
Eunice Huang
References
Schachner-Nedherer, A. L., Werzer, O., Kornmueller, K., Prassl, R., & Zimmer, A. (2019). Biological activity of miRNA-27a using peptide-based drug delivery systems. International Journal of Nanomedicine, 7795-7808.
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