Enrichment process of S-sulfhydrated proteins from cell lysates using biotin-HPDP
Description
This template depicts the method to enrich S-sulfhydrated proteins from cell lysates. The cells are lysed by passing through a 26-3/4-gauge needle multiple times. The thiol groups of free cysteines are blocked using MMTS. Unused MMTS is removed by protein precipitation using acetone. The proteins are resuspended and labelled using biotin-HPDP. The biotin-labelled proteins are pulled down using streptavidin agarose beads, after which the proteins are extracted by denaturation.
Acknowledgements
Anand Kumar Patel
References
Paul, B. D., & Snyder, S. H. (2015). Protein sulfhydration. Methods in enzymology, 555, 79–90. https://doi.org/10.1016/bs.mie.2014.11.021
Get started with this template for freeDon’t start from scratch.
Create professional scientific illustrations quickly and easily, even without any design expertise
- Get started with a huge library of editable icons and templates such as common biological pathways, anatomy, or genetics.
- Create figures that effectively communicate your research in half the time using our editable icons.
- Use our PDB tool to quickly generate and customize protein structures
.gif)
