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This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication. This template describes the process of engineering autologous graft in a preclinical porcine model. In particular, it can be used for repairing the ramus-condyle unit of the temporomandibular joint (TMJ). An anatomically precise scaffold is created using image-guided milling technique on a bovine decellularized mandible. The scaffold is then seeded with porcine adipose-derived stromal cells and cultured in a perfusion bioreactor. Finally, the obtained recellularized scaffold is orthotopically implanted in the porcine model. The graft can be explanted for further functional, structural, and molecular analyses.