Double-Selection Strategy vs. Single-Selection Strategy
Description
The figure depicts the double-selection and single-selection strategies for allelic exchange mutants. In the double-selection method (A), a two-step process with a counterselectable marker is employed, enabling the construction of defined unmarked mutants. The single-selection approach (B) involves introducing a selectable-counterselectable cassette into the target gene, resulting in its interruption. Subsequent allelic exchange within the mutated gene leads to the loss of the cassette.
Acknowledgements
References
Reyrat, M., Pelicic, V., Gicquel, B., & Rappuoli, R. (1998). Counterselectable Markers: Untapped Tools for Bacterial Genetics and Pathogenesis. Infection and Immunity, 66(9), 4011-4017. https://doi.org/10.1128/iai.66.9.4011-4017.1998
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