Directed Evolution of Proteins
Description
This template describes the conventional directed evolution of proteins as a powerful method in protein engineering.
A gene of interest is used to generate a DNA library by one of many established methods. The generated library is transformed to E.coli cells, where the protein of interest is expressed. This is followed by screening or selection for the desired function. Selected evolved genes are used as templates for another round of evolution.
Acknowledgements
References
Arnold, F. H. (2018). Directed Evolution: Bringing New Chemistry to Life. Angewandte Chemie International Edition, 57(16), 4143-4148. https://doi.org/10.1002/anie.201708408
Packer, M., Liu, D. Methods for the directed evolution of proteins. Nat Rev Genet 16, 379–394 (2015). https://doi.org/10.1038/nrg3927
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