Differential Scanning Fluorimetry (Data View)
Description
DSF is a spectrophotometric technique that monitors thermally induced protein unfolding. This method utilizes dyes that fluoresce under non-polar environments, including exposed hydrophobic regions of the protein structure.
Acknowledgements
References
Niesen, F., Berglund, H. & Vedadi, M. The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability. Nat Protoc 2, 2212–2221 (2007). https://doi.org/10.1038/nprot.2007.321
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