CRISPR/Cas9 Gene Editing
Description
This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication.
CRISPR/Cas9 is a powerful tool for genome engineering. The Cas9 complex with a sgRNA recognizes a specific sequence, the protospacer. This is only possible if this sequence is followed by a Protospacer Adjacent Motif (PAM). When Cas9 binds, a dsDNA break is generated. Now, Non-Homologous End Joining or Homology Directed Repair can occur, leading to mutations or gene changes, respectively.
Acknowledgements
References
Addgene. (2017) Chapter 3: Using CRISPR in Your Experiments. CRISPR 101: A Desktop Resource.
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