CRISPR Screening Protocol
Description
This protocol template provides a general flow of CRISPR screening methodology. It includes oligo array synthesis, plasmid cloning, lentiviral infection, genome editing, screen selection, sequencing, and data analysis. This workflow can be customized for other CRISPR/Cas9 screening systems.
Acknowledgements
References
https://www.nature.com/articles/nprot.2013.143
https://www.ncbi.nlm.nih.gov/pubmed/28333914
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