CRISPR-mediated Transcriptional Activation
Description
This base template provides a general view of the CRISPR/dCas9 transcriptional activation system. dCas9 protein, in complex with a transcription activator, binds to a specific DNA sequence guided by sgRNA. This CRISPR-mediated system allows regulation of gene expression. This template can be used as a starting point for more complex workflows that uses CRISPR/dCas9 systems as transcriptional activators.
Acknowledgements
References
https://www.nature.com/articles/nprot.2013.143
https://www.ncbi.nlm.nih.gov/pubmed/28639197
https://old.abmgood.com/marketing/knowledge_base/CRISPR-Cas9-dCas9-Gene-Regulation.php
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