CRISPR-Cas9 Gene Editing Process

Description

CRISPR-Cas9 is a new area of biomedical science that enables gene editing. The Cas-9 (CRISPR-associated protein) binds with a SgRNA (single guide RNA), searching for the target DNA with the use of a protospacer. It is only possible if this target sequence is followed by a protospacer adjacent motif (PAM). 3 A double-stranded break. Then, non-homologous end-joining or homology-directed recombination can occur, leading to mutations or changes in the gene sequence.

Acknowledgements

Priya Heng (Creator)

References

Dragt, E. (2020). CRISPR/Cas9 Gene Editing. https://app.biorender.com/biorender-templates/figures/all/t-5f873df466346900a43c6db1-crisprcas9-gene-editing Ronai , I., & Lynch, K. (2019). What 3D printing can teach us about how to regulate CRISPR gene editing. Retrieved from https://geneticliteracyproject.org/2018/02/27/3d-printing-can-teach-us-regulate-crispr-gene-editing/ Sluss, M. L. (2022). Pam Sequence (protospacer adjacent motif) - definition, functions, properties. Retrieved from https://microbiologynote.com/pam-sequence/

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