CRISPR-Cas9 Gene Editing in Trypanosoma cruzi
Description
This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication.
This figure briefly describes one of the CRISPR/Cas9 gene editing methods for rapid generation of knock out populations in Trypanosoma cruzi. In many organisms, double-strand breaks repair can be carried out by two different processes: 1) homology driven repair (HDR) or 2) non-homologous end joining repair (NHEJ). In the case of trypanosomatids, they do not have NHEJ, but have another repair mechanism known as Microhomology-mediated end joining (MMEJ) that generally introduces a deletion.
Acknowledgements
References
Burle-Caldas, G.A.. (2018) Assessment of two CRISPR-Cas9 genome editing protocols for rapid generation of Trypanosoma cruzi gene knockout mutants. International Journal for Parasitology. https://www.sciencedirect.com/science/article/pii/S0020751918300602?via%3Dihub
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