Creation of chimeric mice expressing GFP macrophages and microglia lacking GFP
Description
Heterozygous female CX3CR1 GFP/- mice will be lethally irradiated ablating the peripheral bone-marrow, following the preparation of green fluorescent bone marrow preparation from tibiae/femurs. Female WT BALB/c mice of age 6 to 10 weeks will be irradiated 1,100 cGy, in which 24h after irradiation, the green fluorescent bone marrow will get injected IV. After 6 weeks reconstruction, chimeric mouse are obtained with GFP expressing macrophages and CNS derived microglial cells are lacking GFP.
Acknowledgements
References
Mildner, A. et al. Nat Neurosci 10, 1544–1553 (2007)
Prinz, M. et al. Journal of Clinical Investigation 116, 456–464 (2006)
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