CITE-seq (Cellular Indexing of Transcriptomes & Epitopes by Sequencing) Workflow
Description
This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication.
This figure shows the workflow of CITE-sequencing, a technique to study the transcriptomes and epitopes of single cells. Antibody derived tags (ADTs) are bound directly to a cell surface protein of interest. The tagged cells are encapsulated within droplets containing distinctly barcoded DNA-microbead. Cells are lysed within the droplet to release the mRNA and bound ADTs. These hybridize with the bead oligos and are converted to cDNA. The cDNA from mRNA and antibody oligos are then separated based on size and amplified for library preparation. The libraries are then sequenced via 10x genomics. RNA-seq and proteomics data can be overlapped by the cell barcodes and visualized.
Acknowledgements
References
Stoeckius, Marlon et al. (2017) Simultaneous epitope and transcriptome measurement in single cells. Nature. https://www.nygenome.org/wp-content/uploads/2017/09/stoeckius.pdf
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