Chemical Mutagenesis and Flow Cytometric Sorting of Parasites
Description
ENU-mediated mutagenesis was performed on parasites, followed by FACS sorting to enrich for parasites expressing a higher tdTomato-ATG8 signal.
Acknowledgements
References
Wang, F., Holmes, M.J., Hong, H.J. et al. Translation initiation factor eIF1.2 promotes Toxoplasma stage conversion by regulating levels of key differentiation factors. Nat Commun 15, 4385 (2024). https://doi.org/10.1038/s41467-024-48685-4
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