Bioconjugation of Bioactive Molecules Using Click Chemistry
Description
Figure depicting usage of bioorthogonal "click chemistry" in live cells to label surface with fluorescent probe enabling detection and mapping. In this method, live cells are supplemented with sugars equipped with an azide functional group that later undergoes a cycloaddition reaction with a constrained cycloalkyne ring. As a result, cell surfaces can be efficiently labeled with fluorescent probes and studied at the molecular level under physiological conditions.
Acknowledgements
Elizabeth Lee
References
Agard, N. J.; Prescher, J. A.; Bertozzi, C. R. A Strain-Promoted [3 + 2] Azide–Alkyne Cycloaddition for Covalent Modification of Biomolecules in Living Systems. J. Am. Chem. Soc. 2004, 126 (46), 15046– 15047, DOI: 10.1021/ja044996f
Nwe, K., & Brechbiel, M. W. (2009). Growing applications of "click chemistry" for bioconjugation in contemporary biomedical research. Cancer biotherapy & radiopharmaceuticals, 24(3), 289–302. https://doi.org/10.1089/cbr.2008.0626
https://www.chemistryworld.com/news/explainer-why-have-bioorthogonal-and-click-chemistry-won-the-2022-nobel-prize/4016337.article
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