Bacterial Chemical Transformation
Description
Bacterial Chemical transformation is a widely used method for introducing plasmid DNA into chemically competent bacterial cells. To achieve this, the cells are mixed with the plasmid DNA and incubated on ice for 5-30 min. Following incubation, a brief heat shock in a water bath facilitates DNA uptake. The mixture is then placed on ice before transferring to a recovery medium for transformed cells to proliferate. This laboratory technique is essential for gene cloning, protein expression, and other molecular biology applications. [Adapted from GPT4, 2023]
Acknowledgements
References
https://www.thermofisher.com/ca/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/molecular-cloning/transformation/competent-cell-basics.html
Get started with this template for freeDon’t start from scratch.
Create professional scientific illustrations quickly and easily, even without any design expertise
- Get started with a huge library of editable icons and templates such as common biological pathways, anatomy, or genetics.
- Create figures that effectively communicate your research in half the time using our editable icons.
- Use our PDB tool to quickly generate and customize protein structures
.gif)
