Antibody probing via gold conjugate for transmission electron microscopy
Description
A visualization of antibody probing via gold conjugate for transmission electron microscopy. Gold particles visible as dark dots on ultrathin sections in TEM.
Ultrathin section on TEM grid (left) with primary antigen-specific antibody (e.g rabbit-anti glutathione, faint green), bound to antigen (e.g. glutathione, green)
and with primary-antibody specific gold-conjugated secondary antibody (e.g. goat-anti rabbit, dark gold, conjugated gold particle, gold) bound to primary antibody
Acknowledgements
References
Koffler et al. 2011. JIPB 53: 930-941. https://doi.org/10.1111/j.1744-7909.2011.01085.x
Zechmann et al. 2008. J Exp Bot 59(14): 4017–4027. doi: 10.1093/jxb/ern243
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