Antibody Probing via gold conjugate

Description

Immuno-gold labeling on embedded sample section surface for subcellular visualization of an antigen. Antigen-specific primary antibody (primary labeled antibody) binds to antigen (e.g. anti glutathione rabbit IgG ), excess primary antibody is washed off; gold-conjugated secondary antibody (e.g. goat anti rabbit IgG) binds to primary antibody in a second labeling step. Gold particles are visualized as electron-dense particles by TEM.

Acknowledgements

Maria Müller Bernd Zechmann Günther Zellnig

References

https://www.researchgate.net/publication/360266173_Organelle-specific_localization_of_glutathione_in_plants_grown_under_different_light_intensities_and_spectra https://link.springer.com/article/10.1007/s00709-010-0111-2 https://pubmed.ncbi.nlm.nih.gov/18977750/

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