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This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication. Agrobacterium-mediated transformation is a widely used method for generating transgenic plants. Key steps include the removal of the T-DNA plasmid from Agrobacterium and cleavage using a restriction enzyme. The desired foreign DNA, cut with the same enzyme, is inserted into the plasmid. The recombinant Ti plasmid is re-introduced into the bacterium, which then transfers the T-DNA carrying the inserted gene into a plant cell chromosome. Culturing these transformed plant cells later results in a transgenic plant expressing the integrated foreign gene. [Adapted from GPT4, 2023]