Agrobacterium-Mediated Transformation
Description
This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication.
Agrobacterium-mediated transformation is a widely used method for generating transgenic plants. Key steps include the removal of the T-DNA plasmid from Agrobacterium and cleavage using a restriction enzyme. The desired foreign DNA, cut with the same enzyme, is inserted into the plasmid. The recombinant Ti plasmid is re-introduced into the bacterium, which then transfers the T-DNA carrying the inserted gene into a plant cell chromosome. Culturing these transformed plant cells later results in a transgenic plant expressing the integrated foreign gene. [Adapted from GPT4, 2023]
Acknowledgements
References
Zhang, X. (2006) Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nature Protocols. https://www.nature.com/articles/nprot.2006.97
Get started with this template for freeDon’t start from scratch.
Create professional scientific illustrations quickly and easily, even without any design expertise
- Get started with a huge library of editable icons and templates such as common biological pathways, anatomy, or genetics.
- Create figures that effectively communicate your research in half the time using our editable icons.
- Use our PDB tool to quickly generate and customize protein structures
![example of using the icon library](https://cdn.prod.website-files.com/621e6f1effebfe03881da9bd/64c7c0fc322ef8c15744f235_ezgif.com-video-to-gif%20(1).gif)