AAV Production by Triple Transfection
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Adeno-associated virus (AAV) production by triple transfection involves the transfection of three plasmids into cells to produce AAV vectors. The first plasmid contains the AAV genome, the second encodes for the AAV Rep and Cap proteins, and the third plasmid provides helper functions needed for the production of viral particles. The transfection results in the replication of the AAV genome and encapsulation into viral capsids, leading to the production of recombinant AAV vectors suitable for gene therapy applications.
[Template description adapted from GPT3.5, 2023]
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