A schematic illustration of the in vitro maturation process in mice

Description

The IVM procedure is performed on secondary follicles with an average size of 100–130 μm. Thus, it is recommended that secondary follicles be collected from 19–23-week-old mice. The obtained follicles are then matured in in vitro culture medium until they become Graafian follicles, called pre-IVM process. Then, denuded oocytes from Graafian follicles will be obtained to perform IVM until reaching metaphase II. For more detailed information, please contact me via email at vothuyanh2202@gmail.com.

Acknowledgements

References

Antonino, D. D. C., Soares, M. M., Júnior, J. D. M., De Alvarenga, P. B., Mohallem, R. D. F. F., Rocha, C. D., Vieira, L. A., De Souza, A. G., Beletti, M. E., Alves, B. G., Jacomini, J. O., Goulart, L. R., & Alves, K. A. (2019). Three-dimensional levitation culture improves in-vitro growth of secondary follicles in bovine model. Reproductive BioMedicine Online, 38(3), 300–311. https://doi.org/10.1016/j.rbmo.2018.11.013 Jessica Greaney, Goutham Narayanan Subramanian, Yunan Ye, & Hayden Homer. (2021). Isolation and in vitro Culture of Mouse Oocytes. https://doi.org/DOI: 10.21769/BioProtoc.4104. Kidder, B. L. (2014). In Vitro Maturation and In Vitro Fertilization of Mouse Oocytes and Preimplantation Embryo Culture. In B. L. Kidder (Ed.), Stem Cell Transcriptional Networks (Vol. 1150, pp. 191–199). Springer New York. https://doi.org/10.1007/978-1-4939-0512-6_12 Pazoki, H., Eimani, H., Farokhi, F., Shahverdi, A., & Tahaei, L. S. (2015). The effects of platelet lysate on maturation, fertilization and embryo development of NMRI mouse oocytes at germinal vesicle stage. Reproductive Medicine and Biology, 15(2), 115–120. https://doi.org/10.1007/s12522-015-0220-x Taghizabet, N., Bahmanpour, S., Zarei-fard, N., Mohseni, G., Aliakbari, F., & Dehghani, F. (2022). Effect of endometrial cell-conditioned medium and platelet-rich plasma on the developmental competence of mouse preantral follicles: An in vitro study. Clinical and Experimental Reproductive Medicine, 49(3), 175–184. https://doi.org/10.5653/cerm.2022.05260 Qian, J., Zhu, R., Yan, R., Long, X., & Guo, F. (2022). Isolation of mouse ovarian follicles for single-cell RNA-seq and in vitro culture. STAR Protocols, 3(3), 101537. https://doi.org/10.1016/j.xpro.2022.101537

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