Immunoprecipitation Protocol

Description

This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication. Immunoprecipitation (IP) is a method that uses antibody specificity to isolate a particular protein (antigen) from a complex sample mixture. When performing an IP reaction, an antibody is added to the sample to capture a target protein, forming an antibody-antigen complex that is isolated upon bead attachment. Once the bead-antibody-antigen complex has been formed, wash steps are used to remove any free antibody or antigen, followed by downstream readouts such as western blot.

Acknowledgements

References

Kaboord, B.. (2008) Isolation of Proteins and Protein Complexes by Immunoprecipitation. 2D PAGE: Sample Preparation and Fractionation (Volume 1). doi: 10.1007/978-1-60327-064-9_27

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